RESUMEN
Intrauterine insemination [IUI] is known as a routine method for infertility treatment. The effectiveness of IUI is not identical in all cases. So in this study to evaluate different methods of IUI in order to increase pregnancy rate, IUI with cleaning cervical canal by swab is compared to IUI without cervical canal cleaning. This study was conducted from 2008/2/1 to 2008/9/30 in Mirza-Kochak-Khan Infertility center. Totally 224 cases were selected for IUI divided into two groups. Group one [n=112] had cervical cleaning with swab before IUI and group two [n=112] was control group. Pregnancy rate were compared in two groups. Two groups were matched regarding age, type of infertility and number of follicles. Pregnancy rate in cervical canal cleaning [group one] and control group [group two] was 15.1% and 9.8% respectively. The difference was not significant. In this study with cleaning cervical canal by swab before IUI there was a non-significant increase in pregnancy rate
RESUMEN
Our aim was to investigate sperm nuclear chromatin condensation and its correlation with semen parameters and vitality test in infertile patients with spinal cord injury [SCI], varicocele, and idiopathic infertility. Sperm chromatin condensation was determined by aniline blue staining in 22 SCI-injured infertile men, 20 with varicocele, and 28 with idiopathic infertility. The results were compared with the semen analysis parameters and the hypo-osmotic swelling test results. Three grades of staining for sperm heads were distinguished: unstained, showing sperm maturity [G0]; partially stained [G1]; and completely stained, showing sperm immaturity [G2]. The total score was calculated as: [G0 * 0] + [G1 * 1] + [G2 * 2]. In all groups, the total staining score was higher than 75%, corresponding to a high degree of immaturity of sperm. Patients with SCI had a less sperm nuclear chromatin condensation and chromatin stability than patients with idiopathic infertility and varicocele [total scores, 98% versus 89% and 88%, respectively; P <.01]. All of the patients had normal hypo-osmotic swelling test results. Sperm counts for all patients were within the reference range. The mean percentages for normal motility and morphology of the sperm were 15.5% and 15% for patients with SCI, 43% and 15% for patients with varicocele, and 62.5% and 54% for patients with idiopathic infertility. There was no correlation between sperm nuclear chromatin condensation and semen analysis parameters. Aniline blue staining for sperm nuclear chromatin condensation is a method independent of semen analysis and demonstrates the internal structural defects of sperm. This method may have a predictive value in assessing fertility